Optimization of clear liquid fermentation condition for ethanol production from Canna edulis Kerl

In this paper, clear liquid fermentation condition for ethanol production from Canna edulis Kerl with orthogonal design was studied. We have studied five factors that influenced clear liquid fermentation condition for ethanol production from Canna edulis Kerl by the single-factor test. The five factors were α-amylase amount, glucoamylase amount, pH, nitrogen and auxiliary materials amount. Selected the best amounts of α-amylase, glucoamylase, pH, nitrogen source and auxiliary materials by means of the singefactors test. And then, we used the Orthogonal test to optimize the fermentation process. The study showed that the best fermentation conditions were α-amylase amount 7 u/g C.eduli, glucoamylase amount 145u/g C. edulis, pH 4.4 and the amount of urea 0.08%, 1% of rice bran as auxiliary materials. The rate of alcohol which was produced by the best fermentation conditions is 21.5% that increased by 1.3% than the rate of alcohol by the single-factor test.